aeromedi
aeromedi

Effective Colouring of Extracted and Isolated Proteins


The staining solution redprot® reveals its features clearly in biochemical use:
- discrimination of protein concentrations over a broad range (see >>Evaluation)
- starting from appropriate sensitivity at low levels
- background free on relevant membranes wtihout intense washing

Staining of purified proteins dot-bloted on Nitrocellulose-Membrane (Hybond)

dots_nitrocellulose_compared Bovine serum albumin

Carboanhydrase

Chemotrypsine A


Egg Albumin


alpha-Lactalbumin


RNase


Streptase


Urease

(5 µg/dot diluted stepwise 1:2)
  redprot®       Coomassie Blue    Amido Black     
(diluted 1:2 with a. dest, stained  for 10 minutes)
 


Staining of purified proteins dot-bloted on PVDF-Membrane (BioRad)

dots_PVDF_compared Bovine serum albumin

Carboanhydrase

Chemotrypsine A

Egg Albumin

alpha-Lactalbumin

RNase

Streptase

Urease
(5 µg/dot diluted stepwise 1:2)
   redprot®        Coomassie Blue       Amido Black
   ready to use ( for 3 minutes)
 


Staining of pollen proteins bloted on Nitrocellulose-Membrane (Protran BA 79, S&S)
after separation of 20 µg/lane by SDS-PAGE (10 to 20%)

    t3    t4     t4   Stdd     t3    t4    t4   Stdd
Elfo_gel_redprot                                     
Elfo_gel_PonceauS                                   
redprot®
(diluted 1:2 with
a. dest for 10 minutes)
Ponceau S

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